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KMID : 0381919980280020171
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Korean Journal of Microscopy
1998 Volume.28 No. 2 p.171 ~ p.180
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A Cytochemical Study on the Vacuolar Apparatus Participating in the Transport of Bile Acids in the Rat Hepatocytes (Cytochemical Study on the Vacuolar Apparatus for Bile Acid Transport)
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Shin Young-Chul
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Abstract
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In the present study, the vacuolar apparatus were investigated in the hepatocytes of rats treated with DA by transmission electron microscopy of conventional and cytochemical thin sections. In the rats after 20 min of dehydrocholic acid treatment, the cis Golgj cisterns were sacculated in line. The saccule occasionally occured by elongation and attenuated neck. The lysosomes also showed protrudent saccule. The vesicles were observed near the cis Golgi cisterns, lysosome and bile canaliculi. Some of the vesicles appeared to be fused to bile canaliculi. The cis Golgi cisterns usually faced toward the bile canaliculi both in normal and experimental groups. The cis Golgi cisterns, protrudent saccule and vesicles were almost devoid of visible contents. The osmium deposits were heavy on the protrudent saccule as well as on the cis Golgi cisterns or on the vesicles isolated near by, but they were light or not observed on the vesicles in the immediate vicinity of bile canaliculi. The acid phosphatase activities appeared on the lysosome and vesicles located near by, but did not appear on the vesicles as approaching closer to the bile canaliculi. The evidence suggests that the vesicles are derived from the cis Gogi cistern and lysosomes and fuse to bile canaliculi for exocytosis, and that the activity in the vesicles is diminished as approaching closer to the bile canaliculi.
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KEYWORD
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Vacuolar apparatus, Golgi apparatus, Endoplasmic reticulum, Lysosome, Vesicle, hepatocyte, Dehydrocholic acid
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